Zinc improves resistance against Alternaria brassicicola infection in Arabidopsis thaliana

سال انتشار: 1395
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 314

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

BIOCONF19_225

تاریخ نمایه سازی: 22 دی 1396

چکیده مقاله:

Zinc is an essential micronutrient for all organisms, including plants and their pathogens. During the infection process, pathogen and host can compete for the essential mineral nutrients and withdrawal of the element by one of the organisms of the pathosystem can cause deficiency in the other. Contrastingly, under conditions of high micronutrient supply a host with high tolerance may use the excess ion as a defence mechanism against the pathogen. This elemental defence hypothesis has been proposed as a driver for the evolution of metal hyperaccumulating plants. However, there are some reports that indicate that metals can also be effective in defence against pathogens in non–hyperaccumulating plant species either directly or in combination with organic defences. To explore this hypothesis in the present work we analysed the influence of two Zn concentrations (2 μM and 12 μM) on the infection of Arabidopsis thaliana by Alternaria brassicicola. The camalexin-deficient mutant pad3-1 and a mutant with impaired Zn vacuolar storage, mtp1-1 were investigated in comparison to the corresponding wild types. The 50% effectconcentration of Zn for inhibition of fungal growth in in vitro experiments was 440 μM. Leaves of the host plant were able to accumulate equivalent concentrations without signs of toxicity. High Zn was unable to substitute for camalexin as the main organic defence compound in A. thaliana. However, surplus Zn enhanced the resistance of A. thaliana to the infection by A. brassicicola in plants that were able to synthesize camalexin: Col-0, mtp1-1 and the corresponding wildtype WS. This increased pathogen resistance was mainly due to a Zn-induced activation of the jasmonate/ethylene signalling pathway leading to enhanced expression of PAD3 the gene coding for the enzyme involved in the last step of camalexin biosynthesis. In conclusion, high, nonphytotoxic Zn concentrations can reinforce plant resistance to fungal infection through activation of defence signalling pathways. An adequate Zn supply is a key factor in plant resistance to fungal infection.Acknowledgements: Supported by BFU2010-14873 and BFU2013-42839R

نویسندگان

S Martos

Lab. FisiologiaVegetal , Fac. Biociencias, Univ. Autónoma de Barcelona, Bellaterra Spain

B Gallego

Lab. FisiologiaVegetal , Fac. Biociencias, Univ. Autónoma de Barcelona, Bellaterra Spain

C Cabot

Lab. FisiologiaVegetal , Fac. Biociencias, Univ. Autónoma de Barcelona, Bellaterra Spain

M Llugany

Lab. FisiologiaVegetal , Fac. Biociencias, Univ. Autónoma de Barcelona, Bellaterra Spain