The Effects of Electromagnetic wave in Radiofrequency Range on the pH Responses of Glutamate Oxidase Based Biosensor

Introduction: Glutamate is an important neurotransmitter in which has an essential role in brain performance. The studies showed that 915 MHz electromagnetic wave – the mobile frequency- can affect the release of glutamate. In this research for measurement of glutamate release a glutamate biosensor is fabricated using immobilizing of glutamate oxidase at the surface of platinum electrode. Since the electromagnetic wave may be affect the immobilized enzyme function, this fact is studied by determination of optimum pH of fabricated biosensor performance. Material and methods: For investigation of 915 MHz electromagnetic wave effect on glutamate oxidase function, the enzyme was irradiated in GTEM cell wave guide for 60 minutes. Then irradiated enzyme and nano chitosan immobilized at the surface of platinum electrode. For determination of optimum pH, the responses of fabricated biosensor at various pH was investigated in buffer phosphate 0.1 M in presence of 0.5 mM glutamate using cyclicvoltametry method with 10 mv/s scan rate. The responses of fabricated biosensor with non-irradiated and irradiated enzymes were compared.Results and conclusion: The results showed that the optimum pH for fabricated biosensor using irradiated enzyme was 5.4, whereas, the optimum pH for fabricated biosensor using native enzyme was 7.4. It seems that the 915 MHz electromagnetic wave can affect the conformation and function of glutamate oxidase.

کلیدواژه ها:

Radiofrequency ، Glutamate oxidase ، Electrochemical Biosensor ، Exposure