Effects of the combination of clove oil and ethanol as an anaexthetic agent on some hepatic enzymes activities in common carp (Cyprinus carpio)

Objective: This study was carried out to examine the effects of a combination of clove oil and ethanol on the blood concentration of some hepatic enzymes in the common carp. Although a body of literature exists about the effects of different anaesthetics on fish, to author’s knowledge there is no information available about clove oil effects on hepatic enzymes activities during and after anaesthesia, which would be important to know especially in aquaculture research. Method & Materials: In the present study, 80 apparently healthy fish weighing between 125-200 g were divided in ten groups: a control group of ten fish, and the experimental groups consisting of ten fish each, which were anaesthetized with 100 ppm of clove oil diluted in pure ethanol at a ratio of 1:9. The blood samples were taken before anaesthesia (control group), 30 min (group one), 60 min (group two), 2 hours (group three), 6 hours (group four), 12 hours (group five), 24 hours (group six), and 48 hours (group seven) after exposure to anaesthetic, respectively. Results & Conclusion: Serum level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were measured. The results showed that although the level of ALT increased after anaesthesia in almost all groups, but this elevation was not significant compared to control group. There was no significant change in serum AST activity in any group. Serum ALP level decreased after anaesthesia in groups 1, 2, and 3 but this reduction was not significant. These findings suggest that clove oil could be an effective anaesthetic for use in this species with no significant effects on hepatic enzymes activities.

کلیدواژه ها:

Anaesthetic ، Clove oil ، Carp ، Hepatic enzyme