Increase of stability and activity enzyme with immobilized on polymeric nanoparticles

Immobilization of cellulase onto polymeric chitosan nanoparticles and characteristics of immobilized enzyme have been studied. The thermal, operational and storage stabilities of an enzyme were improved after they were immobilized on chitosan nanoparticles. The thermal, operational and storage stabilities of an enzyme were improved after they were immobilized on chitosan nanoparticles. The chitosan nanoparticles were applied to protect the activity of free neutral Cellulase. The highest enzyme activity of immobilized enzyme was allowed at pH 8, identical to that of free enzyme, but the range of the optimum pH was broadened. The optimal temperature of immobilized enzyme was 55 °C, and the range of the optimum temperature was also broadened.

کلیدواژه ها:

Cellulase ، Immobilized enzyme ، Chitosan nanoparticles ، Stability ، Activity