Investigation of Efficacy and Cytotoxicity of Prepared and Characterized Laccase Niosomes (span-tween) on MCF7 (breast cancer) and A549(lung cancer)

Laccases (benzenediol:oxygen oxidoreductases, EC (1.10.3.2) belong to the group of blue oxidases and represent the largest subgroup of multicopper oxidases. the most common Enzymes were found from Trametes versicolor. The optimum temperature and ph are determined for laccase. niosomes containing laccase are prepared in optimum conditions by tow metods : mozaffary and film hydration. the best formulations are selected according the size and morphology so encapsulation percent are measured. zeta potential and size distribution of niosomes are measured by Malvern ,nano sizer and zeta sizer.kinetic parameters for both free and encapsulated enzyme by two methods are measured and the results confirmed the immobilized parameters. Cytotoxcicity test was done on two A549(lung cancer) and MCF7(breast cancer) cell lines due to determination of % Ic50.

کلیدواژه ها:

Laccase ، enzyme immobilization ، encapsulation ، kinetic parameters ، cytotoxcity