Low affinity binding of Cyclodextrins to human serum albumin in high cyclodextrins concentration

Cyclodextrins ,CDs - induced conformational changes of human serum albumin, HSA, inphosphate buffer 50mM at pH7 was investigated, using isothermal titration calorimetry(ITC), UV and fluorescence emission spectroscopic methods. The results indicate thatCyclodextrins induces irreversible denaturation of the HSA structure structure in highcyclodextrins concentration. The emission intensity increases suggesting the loss of thetertiary structure of HSA. The results obtained from the ITC data are in agreement with thespectroscopic methods.