Simultaneous determination of epinephrine and uric acid using glass carbon electrode modified with nanoparticles

Epinephrine (EN), is an important compound for the message transfer in the mammalian central nervous system [1], which exists as an organic cation in the nervous tissue and biological body fluids. The determination of epinephrine levels in biological fluids yields important information on its physiological functions. Quantitative determinations of them are significant method for developing nerve physiology, making diagnosis and controlling medicine [2]. Uric acid (UA) isthe primary product of purine metabolism in the human body and it has been shown that extreme abnormalities of UA levels are symptoms of several diseases (e.g. gout, hyperuricemia and Lesch–Nyhan syndrome). Therefore, it is essential to develop simple and rapid methods for thedetermination of these biological molecules in routine analysis. Electrochemical procedures have been developed to determine them based on their electrochemical activities. Noble metal nanostructures like Au, Pt and perovskite have attracted tremendous attention in recent decades, dueto their excellent optical, electrochemical and electronic properties, which are different from those of bulk metal materials. Particularly, due to the unique properties of perovskite nanoparticles, such as good conductivity, useful electrocatalytic and biocompatibility, severalresearchers utilized perovskite nanoparticles to fabricate electrochemical sensors and biosensors [3]. The modified glass carbon electrode with LaMnO3 nanoparticle showed an excellent electrocatalytic activity for EP, and UA oxidations and for accelerated electron transfer between the electrode and the substances. Calibration curves in the ranges 7.5×10-7 to 4.0×10−4 M and 5.0×10-7 to 4.0×10−4 M were obtained for EP and UA, respectively. The lowest detection limits (S/N = 3) were 5.5×10-8 M and 7.5×10-8 M for EP and UA, respectively. The proposed procedure was also successfully applied for the simultaneous detection of EP, and UA in injectable medicine, blood plasma, and urine samples.

کلیدواژه ها:

Epinephrine ، Uric acid ، Modified electrode ، Voltammetry