Microbial degradation of crude oil by bacteriaisolated from oil-polluted groundwater

Besides benefits in some of the biggest industries, crude oil and its products are also responsible for environmental disasters. Because traditional methods for the cleaning of oil hydrocarbon-contaminated environments are expensive and insufficiently effective, bioremediation is one of the most used methods due to its low cost and high efficiency. The aim of this paper is to examine the potential of bacteria isolated from crude oil-polluted groundwater to degrade light crude oil in laboratory conditions. From the polluted groundwater sample, one type of colonies morphology was observed. This strain, named OV1, was identified as Alcaligenes faecalis by using the API test and based on 16SrDNA. The biodegradation experiment shows that the Alcaligenes faecalis strain OV1 was capable of growing in light crude oil-containing liquid mineral medium (5.5; 1.5; 2.5; and 4.5 %, v/v), with maximum optical density after 5 days of incubation. The crude oil degradation rate was 56.5 to %6.48. The results of this research showed the potential of Alcaligenes faecalis OV1 to be used in the bioremediation of crude oil-contaminated environments

کلیدواژه ها:

Alcaligenes ، crude oil pollution ، degradation ، microorganisms