Evaluation of Structured Parameters of Electrospining and Solvant Casting of polyhydroxybutyrate Nano Scaffold for Cartilage Tissue Engineering

As cartilage joint damages are extensively spread in the society and the ability to repair cartilage tissue in body is limited, achieving a suitable method for cartilage regeneration is an issue that has gained the attention of many scholars. For this purpose, scaffold structure and morphology, along with cell culture on it, can be applied to pave the way for a novel method to treat cartilage injuries, osteoarthritis and cartilage repair. In this study, polyhydroxybutyrate (PHB) is selected as the scaffold. Firstly, PHB (6% w/v) solution was prepared using chloroform solvent by employing two solvent and electrospinning methods. For this purpose, porosity of scaffolds was evaluated. In terms of phase studies, morphology, and specifying agent groups, specific characterization devices such as FT-IR were employed.In solvent casting method, porosity percentage was obtained as 89.67, and in electrospinning method, it was obtained as 82.48. Scanning electron microscope (SEM) indicates good attachment of chondrocytes on all scaffolds.

کلیدواژه ها:

nanoscaffold ، Cartilage Tissue Engineering ، Polyhydroxybutyrate ، Scaffolding ، solvent Casting ، Electrospinning