Evaluation of c-Fos , LAT Expression and HTLV-I Viral Load in Adult T cell Lukemia/Lymphoma and HealthyCarriers

  • سال انتشار: 1394
  • محل انتشار: اولین سمپوزیوم بین المللی سرطان نسترن
  • کد COI اختصاصی: NASTARANCANSER01_083
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 778
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نویسندگان

Samaneh Ramezani

Inflammation and Inflammatory Disease Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran

Hadi Kooshyar

Inflammation and Inflammatory Disease Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran

Zahra Mozaheb

Inflammation and Inflammatory Disease Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran

M.Mahdi Akbarin

Inflammation and Inflammatory Disease Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran

چکیده

Human T-lymphotropic virus type 1 (HTLV-I) infects more than 20 million people worldwide. Itis associated with two human diseases; Adult T-cell Lukemia /lymphoma (ATLL) orHAM/TSP. ATLL as an aggressive leukemia/lymphoma is a malignancy with disregulation ofimmune resopnses, cell cycle and signaling pathways. LAT as an adaptor molecule in thecell signalling pathways and c-FOS as an important transcription factor in Immunity, cell cycleand cellular responses might be implicated in ATLL development, therefore the expression ofLAT and c-FOS have been evaluated in this study. The proviral load might be indicatingmonitoring markers for therapeutic efficiency of HTLV-I associated diseases anddevelopment. Eighteen HTLV-I healthy carriers and ninteen patients with ATLL wereassessed for LAT and c-FOS expression and proviral load of in PBMCs using real time PCR,TaqMan method. The data was analyzed by SPSS software. Interestingly, c-FOS did no anyassociation with ATLL development or progression, however, LAT expression and HTLV-Iproviral load was dramatically increased in ATLL patients compare to HTLV-I carriers (pvalue< 0.000), and seems to be involved in HTLV-I associated ATLL progression. Resultsshowed that increased expression of LAT, as a master molecule of Tcell response mayinvolve in malignancy and TCR signalling may help to this reaction. The findings alsoshowed that c-FOS and MAPK pathway were not involved in ATLL development. Accordingto accumulated data increased HTLV-I proviral load is key to the process of leukemogenesisin HTLV-1 carriers

کلیدواژه ها

HTLV-I, Adult T-cell Lukemia /Lymphoma, Proviral Load, LAT, c-Fos

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