Identification of functionally and structurally important residues of HutA protein in vibrio cholera

Vibrio cholerae is a Gram-negative, comma-shaped bacterium. The bacterium's natural habitat is brackish or saltwater. Some strains of V. cholerae cause the disease cholera. V. cholerae is a facultative anaerobe and has a flagellum at one cell pole as well as pili. V. cholerae can undergo respiratory and fermentative metabolism. Iron is essential for the growth of most bacteria, but in nature the element is highly insoluble in an aerobic environment and therefore unavailable to most organisms. Inside the human body, most iron is in the cell in the form of hemoglobin or other iron-containing proteins or is stored as ferritin. Vibrio cholerae, the intestinal pathogen that causes the disease cholera, can acquire iron in two ways. Under low-iron conditions, the organism synthesizes and secretes the siderophore vibriobactin, which binds ferric iron. To survive in such conditions, Vibrio cholerae expresses HutA in its outer membrane. Evidence suggests that HutA is a useful antigen for inclusion in an effective vaccine, hence the identification of its structure and functionally and structurally important residues is very important.

کلیدواژه ها:

Vibrio cholera ، HutA ، Bioinformatic