Determination of Imipramine by Selective Solid Phase Extraction Using Molecularly Imprinted Polymers (MIP) and Liquid Chromatography

The Molecularly Imprinted Polymers (MIP) are polymers possessing specific cavities designed for a target molecule. MIP are synthetic polymers having a predetermined selectivity for a given analyte, or group of structurally related compounds that make them ideal materials to be used in separation processes. In this study new absorbent for molecularly imprinted solid phase extraction of Imipramine from aqueous solution was synthesized using Methacrylic acid (MAA) as functional monomer, Ethylene glycol dimethacrylate (EGDMA) as a cross linker, 2-2'-Azoisobutyronitrile (AIBN) as initiator and chloroform as porogen, causing a non-covalent bulk polymerization. The synthesized MIP and NIP were characterized by FT-IR spectroscopy, UV-VIS spectrophotometer, thermo gravimetric analysis (TGA/DTG) and scanning electron microscope (SEM). The factors that probably influence the solid phase extraction process, such as pH of loading sample, eluent solvent, loading and eluent solvent flow-rate, volume of the loading and eluent solvent and sorbent mass, were investigated. The recovery percent, limit of detection (LOD), limit of quantification (LOQ) and relative standard deviation (RSD %) for human plasma samples by introduced selective solid phase extraction were 94%, 10 ngmL-1, 33 ngmL-1 and 2.8% respectively. Furthermore, the imprinted polymers were successfully applied as a specific solid-phase extractants combined with high performance liquid chromatography (HPLC) for determination of imipramine in the human plasma samples.

کلیدواژه ها:

Imipramine ، Molecularly imprinted polymer ، Solid-phase extraction ، high performance liquid chromatography