Gene delivery by virus-like phospholipid bilayer nanosystem

Nonviral gene transfer systems for human gene therapy applications represent one of the widest fields of chemical, biological, and medical research today. Among all the available structures, Liposome, due to its having a simple and selfassembled structure besides being nontoxic, has been of interest from the beginning. By using the dehydration– rehydration technique, we were able to encapsulate pDNA without using multivalent cations and with high efficiency (98%) into noncationic lipid bilayer vesicles. These liposomes which were composed of dimyristoyl-snglycero- 3-phosphocholine unlike cationic liposomes were nontoxic. The obtained liposome structure was able to protect DNA against nuclease and was completely stable, in a way that even after 6 months, it still kept the pDNA in its structure, and there was a small change in its size (100–150 nm) determined by dynamic light scattering. The images from TEM indicate that these lipids’ bilayer structures have high potency for fusion. These virus-like lipid bilayer vesicles were able to silence GFP gene by transfecting DNA and RNA to GFP stable CHO cell line. Although the transfection efficiency of this system was lower than commercial cationic liposomes (Lipofectamin) and delivery of these carriers into cells needs further studies, the days are not very far from now when these neutral liposomes can be used as the most efficient carriers for protein, RNA, DNA, and drug delivery into human cells.

کلیدواژه ها:

Selfassembly ، Entrapment efficiency ، Artificial virus