Primary root growth, tissue expression and co-expression analysis of areceptor kinase mutant in Arabidopsis

There is no functional annotation for the majority of the several hundreds of receptor-like kinases inplants. A direct way of inferring the function of these proteins is to study the phenotype that results fromloss of function mutants such as T-DNA mutant lines. In this research a function (phenotype) toAt2g37050 gene that encodes a receptor like kinase in Arabidopsis T-DNA line was assigned. Thisphenotype has a shorter primary root length at later stages of development. Transcription study of thegene showed some tissue specificity with more expression level in the root in comparison with othertissues. To study genes co-expressed with At2g37050, ATTED-II web tool was used. It was found thatthe CLASP gene is co-expressed with At2g37050 with a Pearson correlation > 0.6. In kinematic analysisof the difference in root growth, the length between the root tip and the first epidermal cell with a visibleroot hair bulge for 8 day-old seedlings of wild type plants was 1327± 76.50 µm (n=6) and for the mutantplants, was 1109± 72.28. This parameter of the wild type and the mutant plants shows that loose offunction of At2g37050 gene, reduce cell elongation in the elongation zone of root.

کلیدواژه ها:

Arabidopsis ، Mutant ، Receptor Like Kinase ، Root