The effect of different hormones combination and activated carbon on micropropagation of garlic plant (Allium Sativum)
سال انتشار: 1394
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 528
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شناسه ملی سند علمی:
ICDAN01_068
تاریخ نمایه سازی: 28 اسفند 1394
چکیده مقاله:
In order to determine the best hormone treatment for producing callus through tissue culture in garlic plant (Allium Sativum), an experiment was conducted in different levels of IAA, Kin and 2,4-D hormones in normal MS and MS medium with activated carbon, on a factorial experiment in the base of complete randomized design with four repetitions (figure 2). Firstly, the garlic’s embryo meristem cells are sterilized in a 70 degree alcohol for 30 seconds and 1.5% sodium hypo chloride solution for 15 minutes and then are cultured in a basic MS medium at a culture tube. After one month, the explants were collected from root and shoot tip and leaf and cultured in simple MS and MS medium with activated carbon with IAA(1mg/l) + 2, 4.D (1mg/1) and IAA (1.5mg/1) + 2, 4.D (1mg/1) + Kin (1mg/1) and Kin (5mg/1) + 2,4.D (1.5mg/1). Results from normal MS growth culture suggested that percentage of callus formation in explant of root and shoot tip shows the highest rates in IAA(1mg/1) 2, 4-D(1mg/1) hormone treatment including 67.75% and 98.5% in shoot tip and root, respectively. Results of MS medium with activated carbon culture showed that percentage of callus formation in explant of root shows the highest rates in Kin (5mg/1) 2, 4-D (1.5mg/1) hormone treatment which is 91.75%. The biggest size of callus (2.2 cm2) is observed in explant of root in activated carbon and IAA (1.5mg/1) + 2, 4.D (1mg/1) + Kin (1mg/1). For shoot tip explant, the biggest size (1.4 cm2) is observed in simple MS medium and IAA (1mg/1) + 2, 4.D (1mg/1) hormone treatment. The highest volume of callus formed from the root tip explant is observed in IAA (1mg/I) + 2.4-D (1mg/I) in MS medium without activated carbon (2.125 cc). The highest wet weight of callus is related to the root tip explant in IAA (1mg/I) + 2.4-D (1mg/I) and MS medium without activated carbon (1.17 gr). The highest dry weight of callus is related to the shoot tip explant in IAA (1mg/I) + 2.4-D (1mg/I) hormone treatment and MS medium with activated carbon.
کلیدواژه ها:
نویسندگان
Mona Kashanchi
Department of Plant Breeding, Science and Research Branch Islamic Azad University, Tehran, Iran.
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