Multiplex-PCR detection of fumonisin-producing F.verticillioides and F.proliferatum in corn of feedstuff

سال انتشار: 1394
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 627

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شناسه ملی سند علمی:

ASECONF01_046

تاریخ نمایه سازی: 30 بهمن 1394

چکیده مقاله:

Cereals contamination of feedstuff such as corn with mycotoxigenic species of Fusarium is considered as a main source of fumonisin mycotoxin groups which cause worldwide serious diseases in human and animal health. According, in this study, a total of 100 Fusarium isolates were recovered from corn samples collected from 16 dairy farms in Razavy Khorasan Province, Iran, during 2011-2012. Fusarium isolates were identified on morphological and using species-specific PCR. Considering that, early detection of Fusarium species could be very important to prevent mycotoxin contaminating food and feed grains, a Multiplex-PCR assay was designed on the base of four sets of specific primers including: ITS-F/R, Vert1/2, FUM1-F/R and FP3-F/FP4-R which has been developed for the discovery of the two common Fusarium species (F.verticillioides and F.proliferatum) that they can potentially produce fumonisin. In this study, PCR analysis comfirmed 78% of morphological identification to species level as F.proliferatum (53.8%) and 29.5% as F.verticillioides. 42 (53.84%) of total (78) F.verticillioides and F.proliferatum isolated were fumonisin producing out of which 24 (57.14%) were F.proliferatum while 18 (42.86 %) were F.verticillioides. Moreover, we have established the method to discriminate between the two populations F.verticillioides and F.proliferatum, simultaneously enable us to discriminate fumonisin-producing strains from non-fumonisin-producing strains in the corn grains of animal feedstuff.

نویسندگان

Asiyeh Mehrzad

Department of Food Science and Technology, Sabzebar Branch, Islamic Azad University, Iran

Seyed Ali Mortazavi

Department of Food Science and Technology Ferdowsi University of Mashhad, Iran

Masumeh Mehraban Sangatash

Food science and Technology research institute, ACER Mashhad Branch, Iran

Roya Rezaeian Doloei

Department of Agronomy, Mashhad Branch, Islamic Azad University, Mashhad, Iran

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