Improving total carbohydrate determination in extracted Malva Neglecta mucilage by phenol sulfuric assay

Water soluble mucilage usually has a strong and stable structure, so hydrolyzing the structure is done by a strong acid and heating. One of hydrolyzing methods is phenol sulfuric acid assay. In this method phenol solution is used as reagent and sulfuric acid is used as hydrolyzing agent. Heating is usually used for the hydrolyzing procedure. Hydrolyzing extracted mucilage from texture of mallow plant was done by phenol sulfuric method. Hydrolyzing mallow plant extracted mucilage by phenol sulfuric method in boiling temperature takes more than 84 hours. For improving the method autoclave instrument was used for getting 121oc and 11 PSI pressure. Results showed total carbohydrate in extracted mucilage from mallow plant by phenol sulfuric method was 71.57± 2.8.2 respectively.