The Oral Nanovaccine Omp31 Loaded N-trimethyl Chitosan Induces Protection against B. melitensis and B. suis Challenges by Inducing IL-17 Immune Response

Brucella (B) species are the causative agents of brucellosis, the world’s most prevalent zoonatic disease. B.melitensis 31 kDa outer membrane protein (Omp31) is a promising vaccine candidate for development of a subunitvaccine against brucellosis. The aim of this study is survey of immunogenicity property of Omp31 with Freund’s adjuvant(Omp31-IFA) or with TMC nanoparticle (TMC/Omp31); investigation of influence of administration routes on immuneresponse polarization and finally how to Omp31 could protect against Brucella species. The Th2 response wassignificantly higher in mice immunized with TMC/Omp31 in Intraperitoneal (i.p.) route whereas the Th1 response wasincreased in Omp31 injection and TMC/Omp31 nanoparticles in oral immunized mice. Moreover, oral immunizationwith TMC/Omp31 nanoparticles increased sIgA levels in fecal and significant production of IL-17 as Th17. Finally, theoral administration of TMC/Omp31 nanoparticles induced the significant high protection level against B. melitensis andB. suis. The results showed that immunization route has a pivotal role in immune response polarization and protectiveefficiency of Omp31 antigen. Also, TMC/Omp31 when administered orally confers more protection level may be due tothe elicited Th17 response.

کلیدواژه ها:

Brucellosis ، Omp31 ، Oral Vaccine ، Nanoparticles