Effect of l-carnitine on osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells

Mesenchymal stem cells are almost being considered for their therapeutic potential in clinical studies to treat various diseases, disorders, injuries, tissue engineering and repair. To date, many studies have indicated that some agents such as growth factors, trace element and etc. can improve the efficiency of proliferation and differentiation of mesenchymal stem cells. However, efficacy has not been demonstrated in recent late-stage clinical trials. A series of experimental methods including 3-(4,5- dimethylt hiazol -2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) test, ALP activity measurement and Alizarin Red staining were employed to assess the effect of l-carnitin on the osteogenic differentiation of rat adipose tissue derived mesenchymal stem cells. L-carnitin had no significant effects on the osteogenic differentiation of MSCs at all the concentrations tested.

کلیدواژه ها:

Adipose-derived mesenchymal stem cells ، Cell viability ، L-carnitin ، Osteogenic differentiation