In vitro propagation of Gerbera jamesonii cv. Royal soft pink

سال انتشار: 1393
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 1,087

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شناسه ملی سند علمی:

ISOP01_126

تاریخ نمایه سازی: 15 بهمن 1393

چکیده مقاله:

Gerbera is known as an important plant in flower industry, and is considered as a favorite floricultural crop worldwide, ranked fourth among the top cut flowers. This research was conducted to investigate the effects of different concentrations of benzyladenine (BA) [1, 2, and 3 mgL^-1] solely and or in combinations with naphthalene acetic acid (NAA) [0.2 and 0.4 mgL^-1] on the induction, multiplication and proliferation of shoots in Gerbera gamesonii Bolus cv. ‘Royal soft pink’ from shoot tip explants in a Murashige and Skoog (MS) medium. For rooting of shoots three concentrations of NAA (0, 0.5 and 1 mg L^-1) were examined. The results showed that the induction and proliferation of shoot were affected by the combination of BA andNAA. The highest number of shoots (10 shoots per explant) after two sub culture were obtained in MS medium supplemented with 2 mgL^-1 BA and 0.2 mgL-1 NAA. Considering root fresh and dry weights and root length in rooted plantlets it was not shown the significant difference between 0.5 and 1 mg L^-1 NAA for rooting of shoots. The in vitro rooted plantlets wereacclimatized and transferred to greenhouse with 80% success.

نویسندگان

Farzad Nazari

Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran.Department of Horticultural Science, College of Agriculture, University of Kurdistan, Sanandaj, Iran

Pejman Azadi

Agriculture Biotechnology Research Institute of Iran (ABRII), Karaj, Iran. Ornamental Plants Research Institute of Iran (OPRII), Mahallat, Iran.

Morteza Khosh-khui

Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran.