CCR۵, MCP-۱ and VDR Gene Polymorphisms Are Associated with the Susceptibility to HBV Infection

Genetic variants of chemokine and regulatory cytokines play functional roles in chronic HBV infection. The objective of the study, was to evaluate the association between the CCR۵D۳۲, CCR۵-۲۴۵۹A/G, MCP-۱-۲۵۱۸A/G, VDR-APa۱A/C, VDR-Taq۱T/C SNPs and HBV susceptibility, in samples of Iranian populations. The CCR۵D۳۲, CCR۵-۲۴۵۹A/G, MCP۱-۲۵۱۸A/G, VDR-APa۱A/C, VDR-Taq۱T/C polymorphisms were analyzed by polymerase chain reaction and PCR-RFLP using ۱۰۰ chronic HBV infected (HBV) patients, ۴۰ spontaneously recovered HBV (SR) subjects and ۱۰۰ healthy controls (C). Also, serum levels of protein were monitored. The study showed that the existence of CCR۵-۲۴۵۹A, MCP۱-۲۵۱۸G and VDR-CC alleles significantly increased risk of chronic HBV infection. In addition, WtAGCC haplotype had a higher frequency in HBV patients than C and SR groups and might relate to the natural history of the infection. Statistical analysis indicated positive correlations between CCR۵-۲۴۵۹A/G, MCP۱-۲۵۱۸A/G, VDR-APa۱A/C, VDR-Taq۱T/C genotypes and serum levels of the CCR۵, MCP-۱ and VDR in HBV patients. According to the statistical analysis, significant associations with susceptibility to chronic HBV infection was observed with CCR۵-۲۴۵۹A/G, MCP۱-۲۵۱۸A/G, VDR-APa۱A/C, VDR-Taq۱T/C polymorphisms. In addition, no association of the CCR۵D۳۲ SNP with the disease was found.

کلیدواژه ها:

Chemokine ، HBV ، Single nucleotide polymorphism.