Comparative analysis of scolicidal properties of two ecotypes of Leonurus cardiaca: implications for combating echinococcus granulosus

In this study, leaf and flower samples from Khansar and Kerman ecotypes of Leonurus cardiaca were extracted with methanol and ethanol to analyze their phenolic content, antioxidant activity, and scolicidal efficacy against E. granulosus protoscoleces. Total phenolic content analysis revealed higher values in methanolic extracts, with Khansar leaf extracts yielding ۳۷.۶ ±۱.۲ mg GAE/g compared to Kerman's ۳۱.۱ ±۱.۴ mg GAL/g. Total flavonoid content showed a similar trend, with Khansar leaf methanolic extracts producing ۲۱.۶ ± ۱.۱ mg QE/g versus Kerman's ۱۵.۶ ± ۱.۱ mg QE/g. Total flavonol content analysis corroborated these findings, with Khansar leaf methanolic extracts yielding ۷.۵ ± ۱.۰ mg QE/g compared to Kerman's ۶.۶ ± ۱.۱ mg QE/g. DPPH radical scavenging assays demonstrated concentration-dependent antioxidant activity, with Khansar leaf methanolic extracts showing ۶۲.۲ ± ۱.۰% inhibition at ۱۲۰ µg/ml, surpassing Kerman's ۵۹.۰ ± ۱.۲%. Ferric-reducing power assays further supported these results, with Khansar leaf methanolic extracts exhibiting an absorbance of ۰.۴۳۷ ± ۰.۰۰۵ at ۴۰۰ µg/ml. Scolicidal activity against E. granulosus protoscoleces increased with concentration, with the Khansar ecotype achieving ۱۰۰% mortality at ۹۰ mg/ml after ۳۰ min, and at ۱۲۰ mg/ml after ۲۰ min. The Kerman ecotype did not reach ۱۰۰% mortality at ۹۰ mg/ml concentration, but at ۱۲۰ mg/ml, it achieved ۱۰۰% mortality after ۳۰ min. These results indicate that L. cardiaca extracts, particularly from Khansar and Kerman ecotypes, show promise as a potential treatment for E. granulosus infections.

کلیدواژه ها:

Antioxidant activity ، Echinococcus granulosus ، Leonurus cardiac ، Phenolic Compounds ، Scolicidal activity