Short-term effect of zinc oxide nanoparticles (ZnO-NPs) on Carum capticum growth and physiological effects under hydroponic condition

This study investigated the growth and physiological response in medicinal plant Carum copticum exposed to zinc oxide nanoparticles (ZnO-NPs). Treatments of ۰.۱, ۰.۲, ۱, ۵ and ۲۵µM of ZnO-NPs compared with ۰.۲ µM ZnSO۴ (control; Zn form in Johnson solution) were applied in culture media under hydroponic conditions. The plants shoot/root length, and water content, H۲O۲, anthocyanin and activity POD enzyme were measured. The root length was more sensitive to ZnO-NPs than shoot length. The ۰.۲ and ۱ µM NPs increased plant fresh weight by ۹۶.۸ and ۱۱۵.۲%, respectively. The shoot and root water content increased at ۰.۱-۲۵ µM NPs treatments by ۴۰-۵۱% and ۲۶-۳۴% compared to the control group, respectively. The increase in POD enzyme was accompanied by increased length and fresh weight and anthocyanin contents under ZnO-NPs. The ۲۵ µM ZnO NPs (high level) resulted in ۳۶% shoot length, ۴۴% root length, ۳۸% plant fresh weight and ۲۷% anthocyanin decline, while increased ۴۰% shoot water content, ۲۶% root water content, ۱۷% H۲O۲ and ۶۹% POD activity compared with control. Overall, ۲۵ μM ZnO-NPs treatment induced higher damages to the growth parameters due to H۲O۲ increase (oxidative stress) and the failure of the enzymatic and non-enzymatic mechanisms (anthocyanin and POD) to cope with the H۲O۲ enhancement induced by ZnO-NPs.

کلیدواژه ها:

Ajwain ، Nano Metal Oxide ، nano toxicity