Rapid identification and quantification of Campylobacter jejuni by culture and real-time PCR from slaughtered poultry carcasses in Tehran, Iran

Campylobacter species are the main cause of infectious gastroenteritis in human beings. Consumption of poultry meat has been proposed as one of the important sources of campylobacteriosis in human beings. The aim of this study was to investigate the prevalence of Campylobacter jejuni (C. jejuni) in freshly slaughtered poultry in Tehran using culture and molecular methods. It is a descriptive-analytical and cross-sectional study. Fifty samples of whole fresh poultry were randomly collected from supply centres in Tehran from January to February ۲۰۲۴. The standard method of enrichment and cultivation in modified Charcoal Cefoperazone Deoxycholate agar (mCCD) was used to identify Campylobacter spp. DNA was extracted from the confirmed colonies using a commercial kit. Then the real-time PCR method by amplification of the specific region of C. jejuni (ORF-C) was used to determine the C. jejuni. Out of ۵۰ examined samples, ۱۹ samples (۳۸%) showed positive Campylobacter spp. cultures. The results of the Molecular tests showed that C. jejuni was isolated from ۱۳ samples (۲۶%). The results indicated Campylobacter contamination in poultry carcasses in Tehran. Quality control must be improved to reduce contamination in slaughterhouses.

کلیدواژه ها:

Culture method Public health Poultry Campylobacteriosis Real ، time PCR