Bacterial isolation from domestic cats: Detection of biofilm formation and the ica gene

This study was intended to isolate and to identify bacterial species in domestic cats, to determine their capability to form biofilms, and identify the presence of ica genes (icaA and icaB). There were a total of ۲۲ samples collected at three anatomical locations namely; the oral cavity, skin and forepaws. The isolation of bacteria was carried out in the conventional culture media, which was then subjected to phenotypic and biochemical identification and VITEK ۲ automated identification system to identify the species accurately. The findings indicated that the presence of bacteria growth in all samples of the skin and paws (۱۰۰%) and the presence of bacteria in oral samples (۵۴.۵۴%). Various kinds of bacterial species were obtained. Staphylococcus sciuri is the most common (۳۰%), followed by Staphylococcus xylosus (۱۵%). Antibiotic susceptibility test showed that a majority of isolates are susceptible to a wide spectrum of antibiotics though some patterns of multidrug resistance were also observed. Congo Red Agar (CRA) test proved that ۷۰ percent of the isolates were able to form biofilm. At the molecular level, icaA gene was not found in any of the isolates and icaB gene was found in ۷۷.۷% of the samples tested. These results show that domestic cats can be a source of bacteria that are biofilm forming and having antibiotic resistance and this can pose a threat of the spread of the disease to humans as a zoonotic disease carrier.

کلیدواژه ها:

domestic cats ، biofilm ، Antibiotic Resistance ، Zoonosis ، VITEK ۲