Protective Effects of Tannic Acid–Loaded Selenium Nanoparticles against Peritoneal Adhesions via Regulating Oxidative Stress and Inflammatory Gene Expression

Postoperative peritoneal adhesions remain a significant clinical challenge, often leading to serious complications such as chronic pain, intestinal obstruction, and infertility. Evidence indicates that oxidative stress and inflammation are key drivers of postoperative peritoneal adhesion formation. This study investigated the therapeutic potential of intraperitoneal administration of Tannic Acid (TA)-loaded selenium nanoparticles (SeNPs), in a rat model of surgically induced peritoneal adhesions. Thirty male Wistar rats (n=۶ per group) were randomly divided into five groups to evaluate potential anti-adhesion therapies: sham (no adhesion induction), control (adhesion induction without treatment), SeNPs (۱ mg/kg, single intraperitoneal (i.p.) dose after adhesion), tannic acid (TA, ۲۰ mg/kg, single i.p. dose after adhesion), and a combination group (SeNPs + TA at the same doses, single i.p. dose after adhesion). All animals were killed ۷ days later and cecum tissue samples were collected for biochemical and molecular analyses. Biochemical findings revealed a marked attenuation of oxidative stress, as evidenced by a significant decrease in xanthine oxidase activity and malondialdehyde levels in the SeNPs+TA group compared with the control group (p < ۰.۰۵). Gene expression analysis revealed a synergistic anti-inflammatory effect for the SeNPs+TA combination, which induced significantly greater downregulation of key pro-inflammatory mediators (particularly IL-۱β and IL-۶) compared to either monotherapy. At the level of upstream regulation, NF-κB expression followed a similar but non-significant decreasing trend, a finding that may reflect the limited duration of the single-dose treatment protocol. Collectively, these results suggest that tannic acid-loaded selenium nanoparticles exert potent antioxidant and anti-inflammatory actions, effectively targeting the principal molecular pathways involved in postoperative adhesion formation. Therefore, this combinatorial nanotherapeutic approach shows considerable promise as a therapeutic strategy for the management of postoperative peritoneal adhesions.

کلیدواژه ها:

Peritoneal Adhesion ، Selenium nanoparticles ، tannic acid ، oxidative stress ، Inflammatory Gene