<span class="fontstyle۰">Baneh Gum Essential Oil Enhances Doxorubicin Cytotoxicity and Downregulates hsa-mir-۱۸۱a-۵p in Human Gastric Adenocarcinoma Cells

Introduction: Current treatments for gastric cancer (GC) involve chemotherapy, radiotherapy, and surgery, with chemotherapy being the primary method despite its side effects and potential for drug resistance. Some plants are recognized as valuable sources for anticancer drug development, providing therapeutic benefits and reducing side effects economically. This study aims to investigate the anticancer effects of Baneh gum essential oil (BGEO) alone and in combination with doxorubicin on hsa-mir-۱۸۱a-۵p expression in AGS cells as a GC cell line, addressing the significant burden of late-diagnosed gastric cancer. Materials and Methods: After culturing the AGS cells as a GC cell model, they were treated with different concentrations of BGEO (۱, ۲, ۴, ۶, ۸, ۱۰, ۱۲, ۱۴, and ۱۶ μl) to determine the IC۵۰ over a period of ۷۲ hours, with the initial concentration held constant at ۲۵ μg/ml. Subsequently, cell viability, apoptosis, and the expression of the hsa-mir-۱۸۱a-۵p gene were investigated alone and in combination with doxorubicin (۰.۰۲۵ μM). Results: Our results showed that treatment with BGEO causes a significant decrease in cell viability and a significant increase in the apoptosis of AGS cells compared to the control group (p ˂ ۰.۰۵). Also, the results showed that the expression level of hsa-mir-۱۸۱a-۵p was significantly decreased in comparison to the control group (p ˂ ۰.۰۵). The results of investigating the simultaneous effects of BGEO in combination with doxorubicin (DXR) showed that both agents in combination have more effects than single treatment (p ˂ ۰.۰۵). Conclusions: In summary, BGEO significantly reduces cell viability and increases apoptosis in AGS cells, suggesting its potential as a therapeutic agent. The notable decrease in hsa-mir-۱۸۱a-۵p expression indicates its influence on key regulatory pathways in GC. Additionally, this activity was enhanced in combination with DXR. These findings underscore the promise of BGEO in cancer treatment and call for further research into its mechanisms and clinical applications.

کلیدواژه ها:

Gastric cancer ، Baneh Gum Essential Oil ، Cytotoxicity ، Apoptosis ، MicroRNA