In Vitro Protoscolicidal Efficacy of Boswellia Resin Extract and Its Nanoemulsion Against Echinococcus granulosus

Introduction: Cystic echinococcosis (CE), caused by Echinococcus granulosus, remains a significant zoonotic disease with limited treatment options, necessitating the exploration of novel therapeutic agents. This study aimed to evaluate the in vitro protoscolicidal efficacy of Boswellia resin hydroalcoholic extract and its nanoemulsion formulation against E. granulosus protoscoleces. Materials & Methods: Protoscoleces were obtained from liver cysts of infected sheep at Birjand slaughterhouse and treated with serial dilutions (۰.۱%, ۰.۰۱%, ۰.۰۰۱%, and ۰.۰۰۰۱%) of both formulations over varying exposure times (۵ to ۳۰ minutes). Viability was assessed using ۰.۱% eosin staining, and data were analyzed with SPSS ۲۲ software using the chisquare test. Results: The hydroalcoholic extract exhibited protoscolicidal effects only at concentrations above ۰.۰۱%, achieving ۱۰۰% mortality at ۰.۱% after ۳۰ minutes, though effects at lower concentrations were not statistically significant compared to the control (P>۰.۰۵). In contrast, the Boswellia nanoemulsion demonstrated significantly superior protoscolicidal efficacy, achieving ۱۰۰% mortality at lower concentrations and shorter exposure times (e.g. ۰.۱% at ۱۵ minutes and ۰.۰۱% at ۲۰ minutes), with statistical significance confirmed at these levels (P<۰.۰۵). Conclusion: These findings highlight the potential of Boswellia nanoemulsion as a promising natural agent for hydatid cyst treatment due to enhanced bioavailability and efficacy compared to the extract alone.

کلیدواژه ها:

Echinococcus granulosus ، Boswellia resin ، Nanoemulsion ، protoscolicidal ، in vitro