Intravenous glucose tolerance test in Baluchi ewe lambs receiving dietary supplemental selenium-methionine and chromium-methionine

The impact of dietary supplementation of ۱.۵ mg of Selenium/kg of diet as Selenium-methionine (Se-Met) and ۰.۸ mg of Chromium/kg of diet as Chromium-methionine (Cr-Met) and their combination (Se-Cr-Met) on glucose and insulin responses during intravenous glucose tolerance test (IVGTT) was examined on ۲۴ Baluchi ewe lambs (۱۸-۲۰ weeks of age) with ۶ replicates per treatment. Blood samples were collected before (time ۰) and ۲, ۱۰, ۲۰, ۳۰, ۴۵, ۶۰, ۹۰, ۱۲۰ and ۱۸۰ min after IVGTT. The supplementation of Se- and/or Cr-Met resulted in a lower peak glucose concentration at ۲ minutes and a diminished concentration at ۱۸۰ minutes following the infusion (P<۰.۰۵). Additionally, lambs receiving Cr-Met and Se-Cr-Met exhibited lower serum glucose levels at ۱۲۰ minutes in comparison to the control group (P≤ ۰.۰۵). Furthermore, animals consuming diets supplemented with Se-Met and/or Cr-Met showed a reduced area under the curve (AUC) for glucose and insulin at ۱۸۰ minutes following the IVGTT (P<۰.۰۵). The quantity of circulating malondialdehyde, which serves as an indicator of oxidative stress, was reduced in lambs supplemented with Se-Met and/or Cr-Met (P<۰.۰۵). It can be concluded that the supplementation of organic Se-Met and/or Cr-Met enhanced glucose clearance and insulin sensitivity in growing Baluchi ewe lambs, with these effects possibly mediated by a decrease in systemic oxidative stress.

کلیدواژه ها:

Chromium ، Ewe lamb ، glucose tolerance test ، insulin ، selenium