Studying the effect of different concentrations of benzyl amino purine (BAP) in combination with indole butyric acid (IBA) on Chandler walnut (Juglans regia) multiplication under in vitro conditions

The 'Chandler' walnut cultivar (Juglans regia L.) is known for its high yield and superior tolerance to spring frost compared to other evaluated cultivars and genotypes. Through tissue culture techniques, it is possible to propagate uniform and mature plants in a short period and within a limited space. The objective of the current study was to investigate the effect of various concentrations of the plant growth regulator, including benzylaminopurine (BAP) in combination with indole-۳-butyric acid (IBA), on the in vitro shoot proliferation of the Chandler cultivar. Nodal segments were cultured on DKW medium supplemented with BAP and IBA. The experiment was conducted in a completely randomized design (CRD) with three replications. Treatments included three concentrations of BAP: ۰.۵, ۱, and ۱.۵ milligrams per liter (equivalent to ۵۰۰, ۱۰۰۰, and ۱۵۰۰ µL) combined with a constant concentration of IBA of ۰.۱ milligrams per liter (equivalent to ۱۰۰ µL). After four weeks, plantlet height, number of leaves, number of nodes, and number of shoots were assessed. The results indicated that the treatment containing ۱ milligram per liter of ۱ BAP and ۰.۱ milligrams per liter of IBA yielded the best performance across several parameters, including plantlet height, leaf number, and node number. Increasing BAP concentration led to an increase in both the number and length of shoots in the Chandler cultivar. This specific treatment resulted in the highest number of shoots (۳.۵ shoots per explant) and the highest plantlets (۴ cm).

کلیدواژه ها:

Growth regulators ، In vitro cultivation ، Walnut ، تنظیم کننده های رشد ، کشت درون شیشه ای ، گردو