The effect of yeast-extract on the growth and some physiological indicators of Dracocephalum polychaetum Bornm. Cells

Dracocephalum polychaetum Bornm. is a medicinal herb from the Lamiaceae family and is native to Iran, specifically from regions in the southern part of Kerman province. This study aimed to investigate the effects of different concentrations of yeast extract on the growth and physiological responses in the cell suspension of D. polychaetum. After callus induction, suitable callus was transferred to liquid MS medium supplemented with ۰.۲ mg/L BAP, ۲ mg/L NAA, and ۲۰ g/L sucrose and incubated on a gyratory shaker at ۱۲۰ rpm in the dark. Suspension-cultured cells were then treated with ۰, ۲۵, ۵۰, ۱۰۰, ۱۵۰, and ۲۰۰ ppm concentrations of yeast extract during their exponential growth phase (from day ۸ to ۱۳ after sub-culturing). The results showed that the content of flavonol, anthocyanins, malondialdehyde (MDA), hydrogen peroxide (H۲O۲), proline, total antioxidant capacity, and fresh weight in all treated cells had a significant difference from the control. The results indicated significant differences in the content of flavonol, anthocyanins, malondialdehyde (MDA), hydrogen peroxide (H۲O۲), proline, phosphomolybdate, and fresh weight among all treated cells compared to the control. Different concentrations of yeast extract induced various changes in cell metabolism through oxidative stress and stimulated non-enzymatic antioxidant defense systems, leading to increased production of flavonol, anthocyanin, proline, and total antioxidant capacity. Concentrations of ۵۰ and ۱۰۰ ppm yeast extract had the most significant impact on balancing growth and activating the non-enzymatic antioxidant defense system of the cells. Overall, the findings suggest that yeast extract, as a biostimulant, can enhance the defense system of D. polychaetum cells and improve their adaptability to stressful conditions. This underscores the importance of using yeast extract as an effective tool in physiological studies and plant defense mechanisms.

کلیدواژه ها:

Antioxidant defense system ، Cell suspension culture ، Dracocephalum polychaetum ، Yeast extract ، کشت سوسپانسیون سلولی ، سیستم دفاع آنتی اکسیدانی ، گیاه مفرو ، عصاره مخمر