Monte Lukast Sodium selective determination by Solid Phase Extraction using Molecularly Imprinted Polymer and Liquid chromatography

A molecularly imprinted polymer (MIP) was synthesized and evaluated to selectively extraction of Monte Lukast Sodium in human biological fluids. The synthesis of the MIP was performed polymerization sol-gel method using methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker monomer, Azo-bisisobutyronitrile as the primer and Monte Lukast Sodium template as the target drug. FT-IR spectroscopy, UV-VIS spectrophotometry, Thermo Gravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM) were performed to interpret the specific recognition ability, the binding process and the characteristics of MIPs. In this study, solid phase extraction using the imprinted polymer has been optimized with the experimental design approach for Monte Lukast Sodium with regard to the critical factors which influence the molecular imprinted solid phase extraction efficiency such as sample pH, volume of elution solvent, washing solvent and sorbent mass. Batch rebinding capacity of Monte Lukast Sodium was determined from the derived Longmuir isotherm and was found to be ۱۵.۰۴ mg/g¹. The recovery and limit of detection for blood plasma and urine sample were ۹۶% and ۲.۵۰ ugL, respectively. The results showed that, compared with other reports, MIPS synthetized in this study showed high adsorption capacity besides specific recognition ability in human plasma and urine. HPLC analysis has confirmed the high extraction recovery of Monte Lukast Sodium, from plasma and urine samples with use of proposed MIP-SPE system.

کلیدواژه ها:

Molecularly imprinted polymer ، biological sample ، Monte Lukast Sodium ، High performance liquid chromatography