Evaluation of GSTP۱ Gene Polymorphisms in Infertile Men with Azoospermia in Southern Iran

Introduction: Approximately ۳۰% of infertile men experience unexplained infertility, a condition categorized as idiopathic. Several studies have reported a correlation between specific genetic polymorphisms and impaired spermatogenesis in infertile men with idiopathic oligoasthenoteratozoospermia. Genetic factors contribute to ۶۰% of cases categorized as idiopathic. Assessing semen quality serves as a crucial criterion in evaluating male fertility. Materials and Methods: Sampling was conducted at the infertility centre, involving ۶۰ azoospermic patients and ۶۰ healthy individuals. The objective was to investigate and compare the polymorphism status of both groups using polymerase chain reaction and restriction fragment length polymorphism (RFLP) Technique. Results: In the control group, the highest frequency of GSTP۱ gene polymorphisms was observed in individuals with the AA genotype (healthy homozygous) at a rate of ۵۰%. Conversely, the lowest frequency of GSTP۱ gene polymorphism in the control group was associated with the G/G genotype (patient homozygous) at a rate of ۱۵ %. Regarding the patient group, the frequency of GSTP۱ gene polymorphisms was found to be related to the A/A genotype (healthy homozygous) at ۵۰%. The lowest frequency of GSTP۱ gene polymorphism in the patient group was observed in individuals with the G/G genotype (patient homozygous) at ۱۶.۶%. Additionally, within this group, the frequency of patient heterozygotes (A/G) was determined to be ۳۳.۴%. Conclusion: The polymorphism study showed no significant disparity in the frequency of two dominant alleles and mutant alleles between the patient and control groups within the population of infertile Iranian men.

کلیدواژه ها:

Azoospermia ، Infertility ، GSTP۱ ، Polymorphism