Physiological comparison of the separate or interaction effects of thiamine and epibrassinolide on modulating or intensifying some oxidative stress indices in oilseed rape, cultivar Hyola ۴۰۱, under cadmium stress

Cadmium stress induces oxidative stress in plants such as rapeseed. The use of plant growth regulators (PGR) or vitamins (such as thiamine) can help increase resistance to cadmium stresses. In this study, an attempt was made to study the comparative and reciprocal effects of thiamine and ۲۴-epibrassinolide (EBL) on improving growth parameters and antioxidant resistance of rapeseed cultivar Hyola ۴۰۱ under toxic levels of ۲۵۰ and ۵۰۰ micromolar cadmium chloride in the form of ۲۷ treatment codes in a greenhouse environment. The overall results showed that the separate application of thiamine or ۲۴-epibrassinolide had a significant effect compared to other separate elicitors used in most of the measured parameters and improved the antioxidant resistance of the plant under cadmium stress according to the measured indicatorsm, and even reduced the accumulation of cadmium in the shoot. The results obtained from measuring various growth, biochemical, enzymatic and elemental parameters showed that the separate application of thiamine and ۲۴-epibrassinolide improved the growth and antioxidant resistance of plants under toxic treatment, especially ۲۵۰ micromolar cadmium and it was able to alleviate the toxic effects of cadmium stress on rapeseed. For example, the level of membrane peroxidation (MDA and aldehydes) was well reduced by approximately ۵۰% under ۲۵۰ micromolar cadmium treatment. Although in some parameters such as magnesium nutrition, the combined treatment of thiamine and ۲۴-epibrassinolide had a better effect, in some other parameters such as water content and leaf area, the application of treatments improved plant growth under cadmium stress.

کلیدواژه ها:

Catalase enzyme ، Lipid peroxidation ، Magnesium nutrition ، Growth regulators ، Antioxidant resistance ، آنزیم کاتالاز ، پراکسیداسیون لیپیدها ، تغذیه منیزیمی ، تنظیم کننده های رشد ، مقاومت آنتی اکسیدانی