Evaluation of lipoxygenase and glutathione peroxidase genes along with some biochemical traits under salt stress in wild and mutant cultivars of Hashemi rice

Salinity is an unfavorable environmental factor for plants that affects their growth and fertility. Rice, the second most produced grain in the world after wheat, is relatively sensitive to salt stress. To assess the response of the Hashemi rice genotype and its advanced mutant line under stress, root samples were used for biochemical analysis. In this study, an advanced mutant line of Hashemi rice (tolerant to salinity stress) resulting from irradiation with gamma rays and its wild line (variety Hashmi - sensitive to salt stress) were exposed to salt stress in chopped plots. The experiment was conducted using a completely randomized block design with three replications, utilizing a hydroponic solution. The main factor in the experimental design was salinity treatment (۰, ۱۰۰, and ۱۵۰ mM sodium chloride) and sampling time, with two levels. The sub-factor included genotypes. The results showed that while the amount of H۲O۲ increased significantly in both genotypes when exposed to NaCl, it was higher in the wild genotype. The activity of antioxidant enzymes was significantly increased in the advanced mutant line. Additionally, gene expression increased at different concentrations of salinity stress, with higher values observed in the rice mutant line compared to its wild line. Overall, these findings are valuable for identifying and introducing genes specifically involved in salt stress tolerance in rice roots during the seedling stage for use in breeding programs.

کلیدواژه ها:

Stress ، Antioxidant genes ، Mutant genotype ، Stress ، Antioxidant genes ، Mutant genotype