Probabilistic modeling of mechanical properties in thymol-loaded gelatin films for nano wound dressing applications

Gelatin-based films modified to contain thymol represent an exciting new bioactive material class designed for advanced wound dressing applications. Thymol provides favorable antioxidant and antimicrobial activity; however, we need a quantitative understanding of its effect on the ability of gelatin films to bear a load, which will enable the rational design of these products. The goal of this work was to develop a physics-informed probabilistic modeling framework to predict and analyze the mechanical properties (tensile strength (TS), elongation at break (EAB), and Young’s modulus (E)) of thymol-loaded gelatin films as a function of thymol concentration (۰–۸% w/w), using published experimental data. We fit exponential decay and saturating functions to capture the concentration dependent trends, and used a Monte Carlo simulation (۵,۰۰۰ trials) to quantify the uncertainty in the parameters and to estimate the probability of meeting functional mechanical thresholds (TS > ۱.۵ N/m², EAB > ۱۵۵%, E > ۲.۰ MPa). Our work shows a fundamental trade-off effect: thymol provides an increase in flexibility (EAB), yet at the same time diminishes the strength and stiffness. The best range of thymol for the balance of performance is ۲-۴%, at which the likelihood of meeting all three criteria is highest.Gelatin-based films modified to contain thymol represent an exciting new bioactive material class designed for advanced wound dressing applications. Thymol provides favorable antioxidant and antimicrobial activity; however, we need a quantitative understanding of its effect on the ability of gelatin films to bear a load, which will enable the rational design of these products. The goal of this work was to develop a physics-informed probabilistic modeling framework to predict and analyze the mechanical properties (tensile strength (TS), elongation at break (EAB), and Young’s modulus (E)) of thymol-loaded gelatin films as a function of thymol concentration (۰–۸% w/w), using published experimental data. We fit exponential decay and saturating functions to capture the concentration dependent trends, and used a Monte Carlo simulation (۵,۰۰۰ trials) to quantify the uncertainty in the parameters and to estimate the probability of meeting functional mechanical thresholds (TS > ۱.۵ N/m², EAB > ۱۵۵%, E > ۲.۰ MPa). Our work shows a fundamental trade-off effect: thymol provides an increase in flexibility (EAB), yet at the same time diminishes the strength and stiffness. The best range of thymol for the balance of performance is ۲-۴%, at which the likelihood of meeting all three criteria is highest.