Evaluation of the Immunogenic Potential of an Inactivated Pasteurella vaccine and the Role of Titer and Adjuvant Type in Reducing Mortality in Poultry

Background: Pasteurella multocida is a serious bacterium that causes fowl cholera, a contagious and often fatal disease in poultry. It leads to significant economic losses in poultry industry worldwide; thus, it’s recognized that vaccination can be a fundamental strategy. Adjuvants in boosting vaccine potency is a concern that still needs to be examined. Methods: In this study, an inactivated vaccine with defined titer was prepared from the purified bacterial isolate. Meanwhile SPF chickens were randomly assigned to six groups (A-F) and vaccine was administered to these groups in combination with different adjuvants. Also both challenged and non-challenged control groups were included. The mortality rates were recorded up to ۱۴ days post-challenge. Results: The results on days ۱۴,۲۱and۳۵ indicated that administration of vaccine formulated with Adjuvants ۱, ۲ and ۳ (groups B,E and F) notably reduced mortality rates which means no death were observed in the groups receiving the vaccine combined with the local adjuvant, whereas the challenged control group led to the highest mortality. Conclusion: These findings demonstrate that proper formulation of an inactivated vaccine with a suitable adjuvant (potentially Adjuvant۱ in group B) represents an effective approach for preventing mortality associated with Pasteurella in poultry.

کلیدواژه ها:

Adjuvant ، Poultry ، Potency Assay ، Mortality