Isolation of Salmonella from portioned fresh chicken meat and evaluation of antibiotic resistance of the isolates

سال انتشار: 1404
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 2

متن کامل این مقاله منتشر نشده است و فقط به صورت چکیده یا چکیده مبسوط در پایگاه موجود می باشد.
توضیح: معمولا کلیه مقالاتی که کمتر از ۵ صفحه باشند در پایگاه سیویلیکا اصل مقاله (فول تکست) محسوب نمی شوند و فقط کاربران عضو بدون کسر اعتبار می توانند فایل آنها را دریافت نمایند.

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

IVSC13_0216

تاریخ نمایه سازی: 3 اسفند 1404

چکیده مقاله:

Background: Salmonella enterica is a major foodborne pathogen and a prominent cause of infectious gastroenteritis worldwide, primarily linked to contaminated poultry products. It is responsible for an estimated ۹۳ million annual cases of gastroenteritis worldwide, imposing economic burdens estimated in the billions of dollars in the USA and the EU. Poultry is a major reservoir, accounting for approximately ۴۰% of human salmonellosis cases. The emergence of antimicrobial resistance (AMR) presents a critical global health threat. The misuse of antibiotics in poultry farming has fueled this problem, which could lead to millions of deaths by ۲۰۵۰ and prompted the WHO to classify Salmonella as a "priority pathogen". This study aimed to analyze antibiotic resistance in Salmonella isolates from fresh & portioned chicken. Specifically, it aimed to determine the prevalence, dominant serotypes, and antibiotic resistance patterns, focusing on the genetic basis and mechanisms of resistance to key antibiotics, including β-lactams and macrolides. Methods: Samples collected from ۱۵ fresh cut-up chickens were cultured on blood and MacConkey agars. Antibiotic susceptibility was tested by disk diffusion method using streptomycin, penicillin, gentamicin, ceftriaxone, tetracycline, azithromycin, erythromycin, lincomycin, and vancomycin. For Salmonella isolation, ۱g samples were pre-enriched in peptone water, then transferred to Rappaport-Vassiliadis broth, and finally streaked on MacConkey, Salmonella-Shigella, Triple Sugar Iron, and Xylose Lysine Deoxycholate agars. Salmonella-positive isolates were evaluated using antisera for the enteritidis serotype. Results: Out of the ۱۵ samples taken from cut-up chickens, Salmonella was isolated from ۱۰ cases(۶۶.۶%). Among these ۱۰ positive samples, ۴ (۴۰%) were identified as belonging to the serotype enteritidis. In the antibiogram test, resistance to the antibiotics vancomycin and penicillin was observed.

نویسندگان

Fatemehnikou Idjadi

Student of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Semnan, Iran.

Samira Fayazfar

Student of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.

AmirAbbas Lakghasemabadi

Student of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Semnan-Iran.

Fatemeh Zahra Roostaei

Department of Food Hygiene, SRC, Islamic Azad University, Tehran, Iran.

Hoda Shabani

Department of Veterinary immunopathology Sciences, SRC, Islamic Azad University, Tehran, Iran.