The rate of IFNL۳ and MTTP genes polymorphism in β-thalassemia major patients infected with hepatitis C virus treated with ledipasvir-sofosbuvir

The main goal of the present study was to investigate the prevalence of interferon lambda ۳ (IFNL۳) and microsomal triglyceride transfer protein (MTTP) encoding gene polymorphism among thalassemia patients infected with HCV. The frequency of IFNL۳ and MTTP encoding gene polymorphism in ۷۹ thalassemia patients infected with HCV and their correlation with the treatment outcome using ledipasvir/sofosbuvir was investigated. Single nucleotide polymorphism detection confirmed the GT, TT, and GG (rs۱۸۰۰۵۹۱) polymorphism of the synthesized MTTP gene fragment and AT, AA, and TT (rs۸۱۱۳۰۰۷) and TG, TT, and GG (rs۸۰۹۹۹۱۷) polymorphisms of the synthesized IFNL۳ gene fragment. GG was the most frequent allele of rs۱۸۰۰۵۹۱ in both males and females followed by GT allele which was ۱۱.۳۹% in males and ۱۹% in females. The TT allele of rs۸۰۹۹۹۱۷ was the most prevalent (۳۷% in females and ۲۵% in males), and the GG allele was the least frequent. Also, the AT allele was more prevalent than the AA and TT alleles of rs۸۱۱۳۰۰۷. No significant correlation was observed between the SNPs detected and the treatment outcome. The GG allele of the MTTP gene was the most effective allele involved in decreasing the ALT, AST, and ALP enzymes. For IFNL۳ (rs۸۰۹۹۹۱۷) and IFNL۳ (rs۸۱۱۳۰۰۷), the TT and AT alleles were the most effective alleles for the ALP enzyme levels, respectively. It seems that there is a correlation between the frequent alleles detected and liver enzymes.

کلیدواژه ها:

Hepatitis C virus ، Thalassemia ، Single nucleotide polymorphism (SNP) ، IFNL۳ ، MTTP ، ledipasvir-sofosbuvir