The Potential of Serum CTHRC۱ Expression Changes for Early Breast Cancer Detection: A Clinical Investigation

Introduction: This study aimed to evaluate the expression of Collagen Triple Helix Repeat Containing-۱ Protein (CTHRC۱) in the serum of breast cancer patients, addressing the need for non-invasive biomarkers and assessing its diagnostic potential for early detection of breast cancer. Materials and Methods: This case-control study involved ۲۴ newly diagnosed breast cancer patients and ۲۴ healthy controls. RNA was extracted from serum and converted into complementary DNA (cDNA), and the expression levels of CTHRC۱ were measured. The significance of differences in gene expression was analyzed using GenEx software.Results: Most patients exhibited metastasis to the axillary lymph nodes and were classified as stage I. There was no statistically significant difference in CTHRC۱ expression between the breast cancer patients and the control group. The Area Under the Receiver Operating Characteristic Curve (AUC) for serum CTHRC۱ levels was ۰.۶۰۱. The sensitivity and specificity of CTHRC۱ were ۵۴.۱۶% and ۵۵.۱۴ %, respectively.Conclusions: While the findings of this study did not align with those from studies on breast cancer tissue, it is anticipated that the sensitivity, specificity, and AUC of the ROC will improve with an increased sample size. The majority of our patients had axillary metastasis and were in stage I. Previous studies suggest that CTHRC۱ expression is elevated in advanced stages of metastasis, both in serum and tissue. To establish the diagnostic value of CTHRC۱, larger-scale clinical studies with more participants are warranted.

کلیدواژه ها:

breast cancer ، Neoplasm metastasis ، Diagnosis ، Tumor biomarker ، Gene Expression Regulation Neoplastic ، Collagen Triple Helix Repeat Containing-۱ Protein