Statistical Optimization of CYP۱۲۴ Expression: A New Benchmark for Mycobacterial Cytochrome Engineering

The study by Gunawan and colleagues (J Appl Biotechnol Rep. ۲۰۲۵;۱۲(۳)) introduces a statistically grounded optimization strategy for the heterologous expression of Mycobacterium tuberculosis CYP۱۲۴ in Escherichia coli using the Box–Behnken Design (BBD). The research identifies the optimal combination of FeCl₃, δ-aminolevulinic acid (۵-ALA), and IPTG for maximizing yield and purity of CYP۱۲۴, a cytochrome P۴۵۰ enzyme implicated in cholesterol metabolism and drug biotransformation. This commentary highlights the scientific significance, methodological robustness, and biotechnological implications of their findings, particularly in the context of tuberculosis (TB) drug discovery and enzyme engineering.

کلیدواژه ها:

Cytochrome P۴۵۰ ، CYP۱۲۴ ، Mycobacterium tuberculosis ، Box-Behnken design ، Enzyme Engineering