Effect of seed priming with chitosan on some germination components and biochemical traits in safflower cv. Goldasht (Carthamus tinctorius L.) seedlings under salinity stress

To investigate the effect of seed priming with chitosan on germination components and biochemical traits in safflower under salinity stress, a factorial experiment was conducted using a completely randomized design at the University of Mohaghegh Ardabili in ۲۰۲۴, with three replications. Treatments included four salinity levels (۰, ۵۰, ۱۰۰, and ۱۵۰ mM) and four levels of chitosan (۰, ۱, ۲, and ۳% by weight volume), all dissolved in ۱% acetic acid. The results showed that salinity stress reduced the germination indicators. However, priming with different levels of chitosan, especially the ۳% level, improved these traits. The total protein content in the treatment with chitosan ۳% and without salinity compared to the control (distilled water) and ۱۵۰ mM salinity showed an increase of about ۸۱%. The amount of soluble sugars in priming with chitosan ۳% compared to the control (distilled water) showed an increase of about ۴۸%. The maximum amount of malondialdehyde (MDA, ۴۱.۵۳۳ nanomol/g FW) was related to the control treatment (distilled water priming) and ۱۵۰ mM salinity. With increasing salinity, the amount of proline increased, but the use of chitosan with a concentration of ۳% increased this trait about ۷۷% compared to the control (distilled water). In general, the use of different levels of chitosan strengthened the physiological and biochemical responses of weak safflower seeds under salinity stress.

کلیدواژه ها:

Chitosan ، Prolin ، Protein ، Sodium chloride ، Soluble sugars ، پروتئین ، پرولین ، قندهای محلول ، کلرید سدیم ، کیتوزان