A Sensitive Analytical Approach for the Spectrophotometric Detection of Sulfamerazine in Its Medicinal Formulations Utilizing N, N-Dimethyl-P-Phenylenediamine Dihydrochloride as a Coupling Agent

One aim of the study was to create a spectroscopic method for evaluating the drug sulfamerazine. The current method creates a violet-colored soluble product by employing an oxidative coupling reaction between sulfamerazine (SMZ) solution and N, N-dimethyl-p-phenylenediamine dihydrochloride solution (N, N-DMPPDADH) in basic medium with sodium periodate (NaIO۴) as an oxidizing agent. A colored product demonstrates maximum absorption at a wavelength of ۵۶۴ nm. The concentration range of ۳.۱۲۵ - ۵۰ µg/mL of sulfamerazine is covered by the technique under evaluation, based on Beer's law. The sensitivity of the technique is shown by the specific molar absorbance value of ۹۳۴۶.۷۷ L/(mol.cm) and the Sandell significant value, or sensitivity per unit length of ۰.۰۲۷۱ μg/cm۲. The study is shown to have exceptional precision, which is corroborated by the low relative standard deviation value of ۰.۷۹%. This method's validated detection limit of ۰.۰۱۲۴ μg/mL demonstrates how accurate it is in recognizing sulfamerazine at such low amounts. The method that was created has been beneficial in sulfamerazine screening for medicinal items. The reported ۹۹.۹۸% recovery rate of sulfamerazine in the studied samples indicates that the approach was successful in precisely determining the sulfamerazine content of real-world samples.

کلیدواژه ها:

Spectrophotometric ، Sulfamerazine ، sodium periodate ، N ، N-DMPPDADH ، Oxidation coupling