Cytotoxic Effects of Methanolic and Ethanolic Extracts of Chlorella vulgaris, Scenedesmus obliquus, and Nostoc on Human Gastric Cancer Cell Line

Background: Gastric cancer is among the most frequently diagnosed and deadliest cancers worldwide. Current treatments often exhibit limited efficacy and are associated with considerable side effects. Objective: This study aimed to evaluate the effects of methanolic and ethanolic extracts of Chlorella vulgaris (CV), Scenedesmus obliquus (SO), and Nostoc (NSC) on apoptosis in the AGS gastric cancer cell line. Materials and Methods: The cytotoxic effects of the extracts at various concentrations were assessed using the MTT assay. Total RNA was extracted to measure BAX gene expression using quantitative real-time PCR. Results: Extracts from all three microalgae enhanced cell death in the AGS cell line. Notably, the ethanolic extract of NSC significantly upregulated BAX gene expression. Conclusion: The ethanolic extract of NSC shows promising anticancer potential and may serve as a candidate for future gastric cancer therapies.

کلیدواژه ها:

Chlorella vulgaris ، Gastric cancer ، Nostoc ، Scenedesmus obliquus