Enhancing Phalaenopsis Orchid Mass Regeneration: Evaluating Culture Medium Efficiency

The Phalaenopsis orchid holds the largest share of the global orchid market and contributes ۸.۵% to the floriculture trade, offering significant economic potential for developing countries. Cost-effective mass propagation methods are essential to meet the increasing demand for in-vitro Phalaenopsis plants. A particularly efficient method involves generating plantlets from flower stalks. This study evaluates the effectiveness of four culture media in producing plantlets from Phalaenopsis flower stalk buds: Vacin & Went (V&W) medium supplemented with ۵ mg•L⁻¹ ۶-benzylaminopurine (BAP) and ۱۵% coconut water (CW); New Dogashima Medium (NDM) with ۱.۴ mg•L⁻¹ adenine hemi-sulfate; Modified Hyponex/Kyoto medium containing ۵ mg•L⁻¹ ۶-benzyladenine (BA) and ۵ mg•L⁻¹ potato juice (PJ); and a control group using half-strength Murashige and Skoog (MS) medium. Parameters such as plantlet count, leaf count, and phenolic secretion were analyzed. NDM exhibited the lowest phenolic symptoms at the base of the node explants, whereas the half-strength MS medium showed the highest phenolic secretion. The Hyponex/Kyoto medium yielded the highest average number of plantlets (۱۷ per node) and leaves (۳۲), surpassing all other media. Given the increased production of both plantlets and leaves from each stem node, Hyponex/Kyoto medium is considered the most effective for large-scale Phalaenopsis clone production, optimizing the propagation process for commercial cultivation

کلیدواژه ها:

Clone ، mass propagation ، Protocorm-like bodies (PLBs) ، Somatic plantlets ، Stalk nodes