Molecular and parasitological assessment of Theileria species and tick vectors in sheep (Ovis aires) from Kerman, Southeastern Iran

This study assessed the prevalence of Theileria species in sheep from Kerman, Southeastern Iran, using semi-nested PCR. Fifty blood samples were collected from the jugular vein of sheep, prepared for microscopic examination, and stored at −۲۰ °C for molecular analysis. Simultaneously, ticks were collected and identified. Molecular diagnostics detected Theileria DNA in ۴۴% (۲۲/۵۰) of samples, all identified as Theileria ovis, compared to ۲۰% prevalence detected by microscopy, demonstrating the superior sensitivity of molecular methods. The tick Hyalomma anatolicum was confirmed as the primary vector. The high prevalence of pathogenic T. ovis emphasizes the importance of molecular surveillance and targeted control strategies to reduce the impact of theileriosis on sheep health and productivity in the region.

کلیدواژه ها:

Theileria ، Sheep ، Semi-nested PCR ، Kerman ، Iran