In Silico genomic fingerprints of the pathogenic cyanobacterial strains isolated from Alma-Gol Wetland (Golestan Province, Iran), using highly repeated sequences

Alma-Gol Wetland (Golestan Province, Iran) is a permanent habitat for the cultivation of many fish and aquatic organisms and is a popular destination for many travelers during the warm seasons of the year. The discovery of several dead fish carcasses on the water surface indicated the presence of cyanotoxins in the said area. Therefore, the present study, is aimed to screen potentially toxic cyanobacterial strains in this wetland by amplifying mcyG, mcyD, and mcyE toxic genes, as well as structural genes including ۱۶S rRNA and ITS. It was also employed the rep-PCR technique to amplify highly repetitive genes HIP, STRR, and ERIC. The results obtained from the dendrogram based on the amplification of palindromic primers showed that, strains Nostoc sp. ۹, Aliinostoc sp. ۱, and Desmonostoc sp. ۱۰ clustered together, while strains Calothrix sp. ۷ and Neowestiellopsis sp. ۲ mostly formed separate branches. However, only the strain Neowestiellopsis sp. ۲ contained the toxic gene mcyD. Furthermore, comparative analysis of the ITS region length revealed that, both the length and the structure of the D۱-D۱ and BOX B helices differed from those of other strains. This study is among the first to demonstrate that fingerprinting techniques can provide new insights into different cyanobacterial strains.

کلیدواژه ها:

dendrogram ، Molecular Phylogeny ، palindromic primers ، Phylogenetic tree ، toxic cyanobacteria