Phylogenetic study of Adonis in Iran using nuclear and plastid DNA data

The genus Adonis is predominantly distributed in the temperate regions of the Northern Hemisphere. The present study is aimed to elucidate the phylogenetic relationship within the Iranian species of the genus Adonis. A molecular phylogenetic approach, based on nrDNA ITS and cpDNA trnL-F sequences, was applied to six species, one subspecies, and three varieties. Phylogenetic analyses were conducted using parsimony and Bayesian inference methods implemented in MEGA and PAUP softwares. The results confirmed the monophyly of Adonis sect. Adonis, consistent with previous studies. In the phylogenetic tree inferred from nrDNA ITS data, species of Trollius clustered together with the perennial species of Adonis (sect. Consiligo) in a single clade, whereas seven taxa of Adonis (sect. Adonis) formed a separate clade. In contrast, the cpDNA trnL-F data revealed that, all the species of Adonis (sect. Adonis and sect. Consiligo) grouped together in a single clade, while the perennial species of Trollius formed a distinct clade. In this analysis, the two clades were separated with ۹۷% bootstrap support. Furthermore, the taxonomic position of A. globosa as a subspecies of A. microcarpa and A. microcarpa as a distinct species was confirmed.

کلیدواژه ها:

Bayesian inference ، molecular systematics ، nuclear ribosomal DNA ، parsimony analysis ، Ranunculaceae ، sequence variation