Resveratrol shows Anti-tumor Effects on AGS Gastric Cancer cell line by Inducing Mitochondrial Apoptotic Pathway

Background: Gastric cancer is one of the most common types of malignancies with a high mortality rate. Treatment approaches for this disease include surgery and chemotherapy, which are associated with high complications and low efficacy. Therefore, finding new therapeutic or adjuvant approaches is of great importance. This study aimed to investigate the effect of resveratrol (RSV) on the AGS gastric cancer cell line. Methods: After preparing and culturing AGS cells, they were treated with different concentrations of RSV for ۴۸h. Then, cell viability and apoptosis rate were studied using MTT assay and flow cytometry technique, respectively. Moreover, after RNA extraction, cDNA synthesis and primer design, the expressions of bax, bcl-۲, casp۳ and p۵۳ genes were studied using RT-PCR technique. Results: RSV showed cytotoxic effects on AGS cells in the MTT assay and decreased cell viability. In addition, cells exposed to ۵۰ and ۱۰۰ μg/mL of RSV showed a significantly higher apoptosis rate compared to control cells. Finally, RSV overexpressed the bax, p۵۳, and casp۳ genes and downregulated the bcl-۲ in AGS cells. Conclusion: Resveratrol is able to induce anticancer effects in gastric cancer by inducing apoptosis through the activation of mitochondrial apoptotic pathway and has the potential to be used as a therapeutic or adjuvant treatment option in gastric cancer patients.

کلیدواژه ها:

Resveratrol ، Gastric cancer ، Apoptosis ، Gene ، Expression