Isolation and Determination of Antibiotic Resistance Pattern of Bacterial Agents of Subclinical Mastitis in Cows of Masal City, Guilan

Background and aim: Mastitis is the most costly disease threatening the cow breeding industry. Early diagnosis of clinical mastitis cases is the best option to prevent cows from getting mastitis. The aim of the present study was to investigate the prevalence and determine the antibiotic resistance pattern of bacterial agents causing subclinical mastitis in dairy cows of Masal. Materials and Methods: In total, ۱۰۰ samples of raw milk were collected from cow farms in Masal city. The samples were evaluated and scored using the California mastitis test. The positive samples collected in each milking session were transferred to the laboratory at the frozen condition and microbial culture and antibiogram were performed on them. All data were statistically analyzed using SPSS software version ۲۵. Results: Results showed that the prevalence of subclinical mastitis was about ۶۷%. The distribution of the most common bacteria isolated from milk samples included: Staphylococcus aureus with ۲۶.۹%, Escherichia coli with ۱۶.۴%, coagulase-negative Staphylococcus with ۱۰.۴%, Streptococcus agalactiae with ۱۰.۴%, Streptococcus dysgalactiae with ۹% and Enterobacter with ۷.۵%. Also, ۷۱.۴% of coagulase-negative Staphylococcus isolates and ۶۶.۷% of Staphylococcus aureus isolates were resistant to penicillin. Conclusion: In general, all bacterial agents isolated from the studied samples were sensitive to ciprofloxacin. Totally, milk produced from cows with subclinical mastitis transmits antibiotic-resistant microbes to humans and leads to the spread of antibiotic-resistant microbes in humans. Therefore, the prevention and treatment of subclinical mastitis in cows needs more attention.

کلیدواژه ها:

Mastitis ، Antibiotic resistance ، Cattle ، Masal ، ورم پستان ، مقاومت آنتی بیوتیکی ، گاو ، ماسال